|
KMID : 0381120170390090975
|
|
Genes and Genomics
2017 Volume.39 No. 9 p.975 ~ p.985
|
|
|
The Brachypodium distachyon methionine sulfoxide reductase gene family
|
|
Gao Yankun
Zhu Jiantang
Ding Pengcheng
Chen Fanguo
Xia Guangmin
|
|
|
Abstract
|
|
|
|
The methionine sulfoxide reductases (MSRs) are a group of thiol-dependent enzymes able to catalyze the conversion of methionine sulfoxide to methionine. Although some plant MSRs are known to act as protectants against various abiotic stresses, their activity in the model grass species Brachypodium distachyon has not been characterized as yet. Here, six B. distachyon MSR (BdMSR) genes have been isolated; they generate eight distinct cDNAs, since two of them (BdMSRB1 and -B5) produce a pair of alternatively spliced messages. The genes were transcribed in the root, culm, leaf and during various stages of caryopsis development. Those induced by abiotic stress (salinity, drought, low temperature, CdCl2, H2O2 and abscisic acid) harbored known stress-responsive cis elements in their promoter sequences. The heterologous expression of five of the BdMSRs (-A2, -A4, -B1.1, -B3 and -B5.1) in yeast revealed that their products gave a measure of protection against salinity, mannitol and oxidative stress. Substrate specificity analysis revealed that BdMSRB1.1 could reduce free Met-R-SO to Met. The enzymatic activities of BdMSRA4, -B1.1 and -B5.1 in transformed yeast under salt treatment have checked and increased obviously resulting in reducing more Met-SO to Met including the peptide and the free types under salt stress than those in control.
|
|
|
KEYWORD
|
|
|
Brachypodium distachyon, Methionine sulfoxide reductase, Saccharomyces cerevisiae, Abiotic stress, Oxidative stress, Enzymatic activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|